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We have investigated the concentration and correlation between the macro and micro-elements found in an herbal plant named Ocimum sanctum (Tulsi) leaf, using Particle-Induced X-ray Emission (PIXE) spectroscopy. The leaf area was analyzed with a 2 MeV scanning proton micro-beam with a spot size of ~ 1 square micrometer. This study is focused on exploring the correlation between the elemental maps generated using X-ray spectra with micro-PIXE. Two types of correlations i.e., elemental, and concentration-phase correlations were examined. The elemental maps are used to find the relation between the spatial distribution of the elements present in the scanned region while the correlation maps help in understanding which phase corresponds to the region of selected concentration ratios. All the elemental concentrations were determined with the detection limits in ng/mg. The analysis of macro-elements showed that the potassium concentration was highest and phosphorus exhibited the lowest concentration whereas iron was found to be highest in the category of trace or microelements. Moreover, broad-beam runs were also performed on the samples to examine the trend for elemental concentrations.